Composite
Part:BBa_K944015:Design
Designed by: Natasha Gómez Group: iGEM12_Panama_INDICASAT (2012-09-26)
Cyanide insensitive terminal oxidase cioAB and cyanide biosensor expression platform
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2441
Illegal XhoI site found at 2329 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 870
Illegal NgoMIV site found at 1427
Illegal NgoMIV site found at 1779
Illegal NgoMIV site found at 2754
Illegal NgoMIV site found at 2995
Illegal NgoMIV site found at 3136
Illegal NgoMIV site found at 3378
Illegal AgeI site found at 1735
Illegal AgeI site found at 2287
Illegal AgeI site found at 5430
Illegal AgeI site found at 6210
Illegal AgeI site found at 6322 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 560
Illegal BsaI.rc site found at 3343
Illegal BsaI.rc site found at 3652
Illegal BsaI.rc site found at 5138
Illegal SapI.rc site found at 3418
Design Notes
We are working very hard with this construct for further characterization.
Source
The cyanide inducible promoter come from Pseudomonas pseudoalcaligenes and the other genes are well characterized biobribricks from the registry.