Composite

Part:BBa_K944015:Design

Designed by: Natasha Gómez   Group: iGEM12_Panama_INDICASAT   (2012-09-26)


Cyanide insensitive terminal oxidase cioAB and cyanide biosensor expression platform


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2441
    Illegal XhoI site found at 2329
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 870
    Illegal NgoMIV site found at 1427
    Illegal NgoMIV site found at 1779
    Illegal NgoMIV site found at 2754
    Illegal NgoMIV site found at 2995
    Illegal NgoMIV site found at 3136
    Illegal NgoMIV site found at 3378
    Illegal AgeI site found at 1735
    Illegal AgeI site found at 2287
    Illegal AgeI site found at 5430
    Illegal AgeI site found at 6210
    Illegal AgeI site found at 6322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 560
    Illegal BsaI.rc site found at 3343
    Illegal BsaI.rc site found at 3652
    Illegal BsaI.rc site found at 5138
    Illegal SapI.rc site found at 3418


Design Notes

We are working very hard with this construct for further characterization.


Source

The cyanide inducible promoter come from Pseudomonas pseudoalcaligenes and the other genes are well characterized biobribricks from the registry.

References